Catalog # MX3835


phospho-Myosin IIA Heavy Chain (Ser-1943)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Nonmuscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. NMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylates NMHC-IIA on Ser-1916 in the C-terminal region and NMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 in the tailpiece and increases disassembly of NMHC-IIA filaments.

 

References
Breckenridge, M.T. et al. (2009) Mol Biol Cell 20:338.
Conti, M.A. & Adelstein, R.S. (2008) J Cell Sci. 121:11.
Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18:3144.
Murakami, N. et al. (1998) Biochemistry 37:1989.
Phospho-NMHC-IIA (Ser-1943) synthetic peptide corresponds to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC.

*For more information, see UniProt Accession P35579
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by Myosin IIA Heavy Chain (Ser-1943) phospho-specific antibody (MP3831) in ELISA, and has been shown to block the reactivity of MP3831 in Western blot. In addition, the peptide is recommended for use in blocking MP3831 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY