Catalog # MX3465

phospho-Myosin Light Chain (Ser-1) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Both smooth muscle and non-muscle myosin II activity is regulated by the phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19 activates myosin II motor activity and increases myosin filament stability. This activation has important roles in various cell motile processes. By contrast, other phosphorylation sites on MLC may inhibit myosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction with actin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the major inhibitory site since Ser-1 is phosphorylated during PDGF-induced stress fiber disassembly and expression of unphosphorylatable MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition of myosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.

 

References
Komatsu, S. & Ikebe, M. (2007) Mol. Biol. Cell 18:5081.
Tan, J.L. et al. (1992) Annu. Rev. Biochem. 61:721.
Sellers, J.R. (1991) Curr. Opin. Cell Biol. 3:98.


Phospho-MLC (Ser-1) synthetic peptide corresponding to amino acid residues surrounding serine 1 in bovine myosin regulatory light chain 9 (Myl9). This peptide sequence is highly conserved in human, rat, and mouse Myl9, and has high homology to the conserved site in other smooth muscle and non-muscle MLCs.

*For more information, see UniProt Accession P19105
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by myosin regulatory light chain (Ser-1), phospho-specific antibody (MP3461) in ELISA, and has been shown to block the reactivity of MP3461 in Western blot. In addition, the peptide is recommended for use in blocking MP3461 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY