Catalog # HX5565


unphosphorylated Histone H4 (Tyr-72)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Chromatin structure is regulated through the activity of core histones (H2A, H2B, H3, and H4) that form the nucleosome. Histone activity is regulated by a variety of post-translational modifications, including acetylation, phosphorylation, and methylation. Histone acetylation and methylation occur primarily at lysine (K) residues in the amino-terminal tail domain. These modifications are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Acetylation and methylation of specific lysine residues creates docking sites for DNA repair, transcription, and chromatin regulatory proteins. Methylation of histones may be regulated by phosphorylation events at sites downstream of the N-terminal tail. In histone H4, both EGFR activation and inonizing radiation induce EGFR nuclear translocation and Histone H4 (Tyr-72) phosphorylation, which creates a docking site for Set8 methyltransferase. This promotes K20 methylation in Histone H4 leading to DNA synthesis and repair.

 

References
Jaskelioff, M. & Peterson, C.L. (2003) Nat Cell Biol. 5:395.
Chou, R.H. et al. (2014) Developmental Cell 30:224.
Unphosphorylated Histone H4 (Tyr-72) synthetic peptide corresponding to amino acids surrounding Tyr-72 in human histone H4. This site is well conserved in rat and mouse histone H4, but the site is not found in other histone family members.

*For more information, see UniProt Accession P62805
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is an unphosphorylated control peptide for HX5555. The peptide is recommended for use in ELISA and for blocking antibody reactivity in western blot and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY