Catalog # GX5795

phospho-Girdin (Ser-1416) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

 

References

Bhandari, D. et al. (2015)Proc Natl Acad Sci. 112(35):E4874
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Simpson, F. (2005) Traffic. 6(6):442



Phospho-Girdin (Ser-1416) synthetic peptide corresponding to amino acid residues around serine 1416 in human Girdin. This sequence is identical in rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.

*For more information, see UniProt Accession Q3V6T2
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by Girdin (Ser-1416), phospho-specific antibody (GP5791) in ELISA, and has been shown to block the reactivity of GP5791 in Western blot. In addition, the peptide is recommended for use in blocking GP5791 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY