Catalog # FX3485


phospho-FHOD1 (Thr-1141)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.

 

References

Takeya, R. et al. (2008) EMBOJ 27:618.
Takeya, R. & Sumimoto, H. (2003) J Cell Sci. 116:4567.
Westendorf, J.J. (2001) J Biol Chem. 276:46453.

Phospho-FHOD1 (Thr-1141) synthetic peptide corresponding to amino acid residues surrounding Thr-1141 in human FHOD1. This peptide sequence is highly conserved in rat and mouse FHOD1, and is well conserved in FHOD3 (Thr-1399).

*For more information, see UniProt Accession Q9Y613
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by FHOD1 (Thr-1141) phospho-specific antibody (FP3481) in ELISA, and has been shown to block the reactivity of FP3481 in Western blot. In addition, the peptide is recommended for use in blocking FP3481 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY