Catalog # EX2845

phospho-Ephexin-1 (Tyr-87) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.

 

References

Zhang, Y. et al. (2007) J Biol Chem. 282(42):31103.
Sahin, M. et al. (2005) Neuron 46:191.
Shamah, S.M. et al. (2001) Cell 105:233.



Phospho-ephexin-1 (Tyr-87) synthetic peptide corresponding to amino acids surrounding tyrosine 87 in mouse ephexin-1. This sequence has significant homology to the conserved site in human and rat ephexin-1.

*For more information, see UniProt Accession Q8CHT1
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This peptide is specifically recognized by phospho-specific Ephexin-1 (Tyr-87) antibody (EP2841) in ELISA, and has been shown to block the reactivity of EP2841 in Western blot and is recommended for blocking in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY