Catalog # DX3025


DISC1 (a.a. 740-753)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Disrupted in schizophrenia 1 (DISC1) is a multifunctional scaffold protein that has important roles in neurodevelopment. DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1 can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with and inhibits the kinase GSK-3β to enable Wnt activation of β-catenin–mediated gene transcription and neuron proliferation. These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 and BBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. During neuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3β is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3β is decreased, and interaction with BBS1 is enhanced during postmitotic neuron migration.

 

References

Brandon, N.J. et al. (2011) Nat Rev Neurosci. 12(12):707.
Ishizuka, K. (2011) Nature. 473(7345):92.
Mao, Y. et al. (2009) Cell. 136(6):1017.

DISC1 synthetic peptide containing amino acid residues 740 to 753 from mouse DISC1. This peptide sequence is well conserved in rat DISC1, but has 7 amino acid differences with the conserved site in human DISC1.

*For more information, see UniProt Accession Q811T9
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-DISC1 (a.a. 740-753) antibody (DP3021 and DP3041) in ELISA, and has been shown to block the reactivity of DP3021 and DP3041 during Western blot. In addition, the peptide is recommended for use in blocking DP3021 and DP3041 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY