Catalog # CX3055

phospho-β-Catenin (Tyr-333) Peptide

Blocking Peptide

Application / Dilution
ELISA50 ng/well

Size 50 μg



β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86, 333, 489, and 654 in β-Catenin. Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin, while c-Abl phosphorylation of Tyr-489 decreases β-Catenin binding to N-Cadherin and leads to nuclear translocation and transcriptional activation. In addition, Tyr-333 phosphorylation is required for binding to the metabolic kinase, PKM2,and promotes nuclear accumulation of both proteins in gliomas.



Yang, W. et al. (2011) Nature. 480(7375):118-22.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.

Phospho-β-Catenin (Tyr-333) synthetic peptide corresponds to amino acid residues around tyrosine 333 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin, and has high homology to the conserved site in γ-Catenin (Tyr-324).

*For more information, see UniProt Accession P35222
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-β-Catenin (Tyr-333) antibody (CP3051) in ELISA, and has been shown to block the reactivity of CP3051 during Western blot. In addition, the peptide is recommended for use in blocking CP3051 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: