Catalog # CX2625

phospho-Coronin-1B (Ser-2) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.

 

References
Cai, L. et al. (2007) Cell 128:915.
Foger, N. et al. (2006) Science 313:839.
Uetrecht, A.C. & Bear, J.E. (2006) Trends Cell Biol. 16(8):421.


Coronin-1B (Ser-2) synthetic peptide corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and coronin-1A. However, this sequence has low homology to the conserved region in other coronins.

*For more information, see UniProt Accession Q9BR76
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This peptide is specifically recognized by Coronin-1B (Ser-2) phospho-specific antibody (CP2621) in ELISA, and has been shown to block the reactivity of CP2621 in Western blot and is recommended for blocking in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY