Catalog # CX1195

phospho-β-Catenin (Tyr-86) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions, leading to changes in cell adhesion.

 

References

Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.

Western blot of β-Catenin immunoprecipitated from A431 cells treated with pervanadate. The blots were probed with β-Catenin (Tyr-86) or β-Catenin (a.a. 649-661) antibodies. The immunoprecipitated β-Catenin was untreated (lanes 1 & 6) or treated with alkaline phosphatase (lanes 5 & 10), and the antibodies were used in the presence of β-Catenin (Tyr-86) (lanes 2,7) or β-Catenin (a.a. 649-661) (lanes 3,8) peptides or BSA-phospho-Tyr (lanes 4,9).



Phospho-β-Catenin (Tyr-86) synthetic peptide corresponds to amino acid residues around tyrosine 86 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.

*For more information, see UniProt Accession P35222
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-β-Catenin (Tyr-86) phospho-specific antibody (CP1191) in ELISA, and has been shown to block the reactivity of CP1191 during Western blot. In addition, the peptide is recommended for use in blocking CP1191 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY