Catalog # CX1135


Cofilin 1 (N-terminus)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.

 

References
Maciver, S.K. & Hussey, P.J. (2002). Genome Biol. 3(5): 3007.
Aizawa, H., et al. (2001). Nature Neurosci. 4(4):367.
Bamburg, J.R. (1999). Annu Rev Cell Dev Biol. 15:185
A synthetic peptide corresponding to amino acid residues at the N-terminus of human Cofilin 1. This sequence has 100% homology to similar regions in rat and mouse Cofilin 1, and has 3 amino acid differences from human Cofilin 2.

*For more information, see UniProt Accession P23528
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Cofilin 1 (N-terminus) antibody (CP1131) in ELISA, and is recommended for use in blocking CP1131 reactivity in Western blot and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY