Catalog # CX1125

phospho-γ-Catenin (Tyr-550) Peptide

Blocking Peptide

Application Dilution
ELISA50 ng/well

Size 50 μg



Plakoglobin (γ-Catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-Catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for γ-Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in γ-Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases γ-Catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases γ-Catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-Catenin protein-protein interactions within desmosomal complexes.



Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391.
McCrea, P.D. et al. (1991) Science 254:1359.

Western blot analysis of anti-γ-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431 cells. The immunoprecipitates were untreated (lanes 1 & 5) or treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-γ-Catenin (Tyr-550) or anti-γ-Catenin (C-terminal). The anti-γ-Catenin (Tyr-550) was used in the presence of γ-Catenin (Tyr-550) peptide (lane 3) or γ-Catenin (Tyr-644) peptide (lane 4).

Phospho-γ-Catenin (Tyr-550) synthetic peptide corresponds to amino acid residues around tyrosine 550 of human γ-Catenin. This peptide sequence is highly conserved in rat and mouse γ-Catenin.

*For more information, see UniProt Accession P14923
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by γ-Catenin (Tyr-550) phospho-specific antibody (CP1121) in ELISA, and has been shown to block the reactivity of CP1121 during Western blot. In addition, the peptide is recommended for use in blocking CP1121 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: