Catalog # AX2045

Atrogin-1 Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.

 

References
Leger, B. et al. (2006) J Physiol. 576(3):923.
Bodine, S.C. et al. (2001) Science 294: 1704-8.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.


A synthetic peptide corresponding to amino acids 23 to 35 in mouse Atrogin-1. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).

*For more information, see UniProt Accession Q9CPU7
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by Atrogin-1 antibody (AP2041) in ELISA, and has been shown to block the reactivity of AP2041 during Western blot.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY