Catalog # CX2165

CRMP2 (C-terminal region) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

References

Cole, A.R. et al. (2006) J. Biol. Chem. 281(24):16591.
Mimura, F. et al. (2006) J. Biol. Chem. 281(23):15970.
Arimura, N. et al. (2005) Mol. Cell. Biol. 25(22):9973.
Uchida, Y. et al. (2005) Genes to Cells 10:165.
Arimura, N. et al. (2000) J. Biol. Chem. 275(31):23973

Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).

CRMP2 synthetic peptide corresponding to amino acids in the C-terminal region of human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has high homology to the conserved site in CRMP1. It has 50% homology to CRMP3 and CRMP5.

*For more information, see UniProt Accession Q16555

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This peptide is specifically recognized by CRMP2 (C-terminal Region) antibody (CP2161) in ELISA, and has been shown to block the reactivity of CP2161 in Western blot and is recommended for blocking in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: