The Wiskott–Aldrich syndrome protein (WASP) family functions downstream of Rho GTPases to mediate the branched-actin network formation required for cytoskeletal remodeling, intracellular transport, and cell motility. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms (WAVE1, 2, and 3). Two new WASP subfamilies are WASH and WHAMM. WASH is an F-actin binding protein that can promote Arp2/3-mediated branch actin nucleation. The WASH protein contains two WASH homology domains, a proline-rich region, and a C-terminal VCA domain. WASH can interact with dynamin, FAM21, and tubulin, and WASH activity may be regulated downstream of Rho GTPases. WASH interaction with FAM21 has been implicated in endosome sorting, and inactivation of WASH leads to endosome tubulation. Thus, WASH may be a critical element in the regulation of the actin filament and microtubule networks that mediate endosomal sorting and fission.
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*For more information, see UniProt Accession A8K0Z3
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Wang, F. et al. (2014) Sci Rep. 7(4):5596. (WB: mouse oocytes)
This kit contains: