Catalog # CP1751

N-Cadherin (a.a. 811-824) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:200
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 130 kDa

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

References

Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.

Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.

unphosphorylated N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids 811 to 824 from human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin.

*For more information, see UniProt Accession P19022

Rabbit polyclonal, affinity-purified antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using N-cadherin (a.a. 811-824) peptide. The purified antibody detects a band at 130 kDa* in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with E-cadherin. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 (WB: PC12-SH2B1? cell)
Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. (WB: human pancreatic cancer PANC-1 and MIA PaCa-2 cells)
Sato, F. et al. (2012) Intern J Molec Med 30:495. (WB: human pancreatic cancer BxPC-3 cells)
Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. (WB: human, bovine VECs)

This kit contains: