Catalog # AM5021


APC (Central region)

Rat Monoclonal

Application Dilution
ELISA1:1000
ICC1:50
WB1:250

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 160/310 kDa

Isotype IgG2a

$245

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and MT association with organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. One group of +TIPs include proteins with basic and serine-rich motifs (SxIP motifs) that mediate interaction between MTs and EB proteins. Adenomatous polyposis coli (APC), MACF, and STM1 are a group of the SxIP motif-containing proteins. APC protein is a large multidomain tumor suppresor protein that has important roles in Wnt signaling, as well as several other cell functions including cell migration, spindle assembly, chromosome segregation, neuronal differentiation, apoptosis, and MT stabilization. APC interaction with EB proteins through its SxIP motif promotes interaction with MTs leading to stabilization and increased polymerization.

 

References
Lui, C. et al. (2012) IUBMB Life. 64(3):209.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1-74.
Clone KT44 was generated from a recombinant protein containing amino acid residues 788 to 1038 from mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2.

*For more information, see UniProt Accession Q61315
Rat monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline and 0.05% sodium azide. Aliquot and Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects full length APC at 300 kDa* and proteolytic fragments of APC at lower molecular weights. The antibody detects APC or its fragments in SW480, CaCO2, DLD-1, and Hct116 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY