Catalog # AM1141

Akt (Ser-473), phospho-specific Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
IP1:100
WB1:250

Size 200 μl

Species Reactivity Hu, Rt, Ms

MW 60 kDa

Isotype IgG1

DATASHEET  




$295

Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

 

References
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Jones, P.F. et al. (1991) Proc Natl Acad Sci. USA 88:4171.

Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).



Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.

*For more information, see UniProt Accession P31749
Mouse monoclonal purified with protein G chromatography is supplied in 200μl phosphate-buffered saline with 0.02% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of mouse NIH3T3 cells treated with PDGF and human A431 cells treated with EGF.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY