Catalog # AM3141

Atrogin-1 M314 Antibody

Rat Monoclonal

Application Dilution
ELISA1:2000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 41 kDa

DATASHEET  




$295

Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.

 

References
Leger, B. et al. (2006) J Physiol. 576(3):923.
Bodine, S.C. et al. (2001) Science 294: 1704-8.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.

Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).



Clone M314 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids 23 to 35 in mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).

*For more information, see UniProt Accession Q9CPU7
Rat monoclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Atrogin 1 peptide (without carrier). This antibody detects a full length human recombinant Atrogin 1 on SDS-PAGE immunoblots.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY