Catalog # WP2601
N-WASP (Tyr-256), phospho-specific
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 65 kDa
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.
Wu, X. et al. (2004) J Biol Chem 279(10):9565.
Torres, E. & Rosen, M.K. (2003) Mol Cell 11:1215.
Baba, Y. et al. (1999) Blood 93:2003.
*For more information, see UniProt Accession O00401
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Uenishi, E. et al. (2013) J of Bio Chem 288(36): 25851. (WB: MIN6-K8 ?-cells)
Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. (WB: mouse adipocytes)
Kalwa, H. & Michel, T. (2011) J Biol Chem 286:2320. (WB: bovine aortic endothelial cells)
Pichot, C.S. et al. (2009) British J Cancer. 1 10. (WB: breast cancer cell lines)
Elias, BC et al. (2015) J Cell Sci. 128(23):4293. (WB: mouse kidney duct cells)
This kit contains: