Catalog # VP2521


Vav2 (a.a. 309-322)

Rabbit Polyclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 100 kDa

$245

The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites, leading to activation of Vav GEF activity.

 

References

Wilsbacher, J.L. et al. (2006) Cell Comm. Signal. 4:5.
Aghazadeh, B. (2000) Cell 102:625.
Schuebel, K.E. et al. (1996) Oncogene 13:363.

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the Dbl homology domain of human Vav2. This sequence has high homology with similar regions in rat and mouse Vav2, and has low homology to other Vav family members.

*For more information, see UniProt Accession P52735
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Vav2 (a.a. 309-322) peptide (without carrier). The antibody detects a 100 kDa* doublet corresponding to the molecular mass of Vav2 on SDS-PAGE immunoblots of human Jurkat, HUVEC, and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-Vav2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-Vav3 (a.a. 293-305) at 1:500 (lanes 4-6).



This kit contains:

KIT SUMMARY