Catalog # VM2771
VASP (C-terminal region) Antibody
Mouse Monoclonal
| ELISA | 1:2000 |
| ICC | 1:50 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu
MW 50 kDa
Isotype IgG1
Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL). These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.
References
Applewhite, D.A. et al. (2007). Mol Biol Cell. 18(7):2579.
Blume, C. et al. (2007) J Biol Chem. 282(7):4601.
Krause, M. et al. (2003) Annu Rev Cell Dev Biol. 19: 541.
Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).
Immunocytochemical labeling of VASP in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal VASP (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
*For more information, see UniProt Accession P50552
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
