Catalog # TP5661

TRPM7 (Ser-1493), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:1000
WB	1:500

Size 100 μl

Species Reactivity Hu

MW 220 kDa

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

References

Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793

Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513), phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497), TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493), phospho-specific (lanes 5 & 6).

Phospho-TRPM7 (Ser-1493) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1493 in human TRPM7. This site is conserved in rat and mouse TRPM7, but the homology surrounding the site is low. This site has no homology to other TRPM family members.

*For more information, see UniProt Accession Q96QT4

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1493) then affinity purified using phospho-TRPM7 (Ser-1493) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells stimulated with calyculin A, and detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: