Catalog # TP4131

α6-Tubulin (Ser-165), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 50 kDa

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

References

Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.

Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKCα (lanes 2, 4, & 6). The blots were probed with anti-α6-Tubulin (a.a. 160-169) (TP4281; lanes 1 & 2), anti-α6-Tubulin (Ser-165) (TP4131; lanes 3 & 4), and anti-α-Tubulin (C-terminus) (TM4111; lanes 5 & 6).

Phospho-α6-Tubulin (Ser-165) synthetic peptide (coupled to KLH) corresponds to amino acid residues around serine 165 of human α6-Tubulin. This sequence is well conserved in most α-tubulin isoforms from most eukaryotic species, but is not conserved in β-Tubulin.

*For more information, see UniProt Accession Q9BQE3

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to unphosphorylated α6-Tubulin (a.a. 160-169) peptide before affinity purification using α6-Tubulin (Ser-165) peptide (without carrier). The antibody detects a 50 kDa* protein corresponding to the molecular mass of α-Tubulin on SDS-PAGE immunoblots of recombinant a1-tubulin phosphorylated with PKCα. This reactivity is not observed after lambda phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: