Catalog # TM4111

α-Tubulin (C-terminus) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:200
IHC1:200
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 50 kDa

Isotype IgG1

DATASHEET  




$195

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

 

References
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.

Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.

Immunocytochemical labeling of α- and βI-Tubulin in rat A7r5 cells. The cells were labeled with anti-βI-Tubulin (TM1541) (left) and anti-α-tubulin (TM4111) (right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 488.



Clone DM1A was generated from purified chick brain tubulin. The antibody recognizes an epitope within the C-terminal end of α-tubulin isoforms. The epitope is highly conserved in α-tubulin isoforms from most vertebrate species.

*For more information, see UniProt Accession Q9BQE3
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 55 kDa* protein corresponding to the molecular mass of α-Tubulin on SDS-PAGE immunoblots of human, rat, and mouse cells and tissues.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY