Catalog # TM1541

β-Tubulin Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:200
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 50 kDa

Isotype IgG1

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

References

Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.

Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.

Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.

Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Immunocytochemical labeling in C2C12 cells using anti-β-Tubulin (TM1541) monoclonal antibody and anti-β-Tubulin (Ser-172) polyclonal antibody. The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of phospho-β-Tubulin (Ser-172) peptide (TX1725).

Clone (M154) was generated from purified porcine brain tubulin.

*For more information, see UniProt Accession Q13509

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 50 kDa* protein corresponding to the molecular mass of β-Tubulin on SDS-PAGE immunoblots of purified brain tubulin, mouse brain tissue, rat PC12 cells, and human A431 and SH-SY5Y cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Salameh, A. et al. (2015) PNAS U S A. 112(27):8403. (WB/ICC: human LNCaP cells)
Wang, Q. et al. (2014) J Virol. 88(9):4853 (WB: HeLa)
Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. (WB: U3&3MG)
Pritchard, J. et al. (2011) Cancer Res 71(17):5850. (WB: mouse lymph node and thymus)
Fagan-Garcia, K. et al (2011) J Virology. 85(2):883. (WB: human HeLa cells)
Campbell, S. et al. (2010) J Biol Chem. 285:4695. (WB: human HeLa cells)
Meacham, CE. et al. (2011) Nature Genetics. 41:1133. (WB: mouse lymphoma)

This kit contains: