Catalog # SP4901

SMAD7 (N-terminal region) Antibody

Rabbit Polyclonal

Application Dilution
WB1:500
IHC1:50

Size 100 μl

Species Reactivity Hu, Rt, Ms, F

MW 46 kDa

DATASHEET  




$245

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

 

References

Attisano, L. & Wrana, J.L. (1998) Curr Opin Cell Biol. 10:188.
Topper, J.N. et al. (1997) Natl Acad Sci. U.S.A. 94(17):9314.

Western blot analysis of SMAD7 in human HepG2 cells. The blot was probed with rabbit polyclonal SMAD7 antibody (SP4901) at 1:250.



This antibody was raised against amino acids 12-29 (RLWRSRAPGGEDEEEGAG) and 36-50 (ELRGEGATDSRAHGA) of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.

*For more information, see UniProt Accession O15105
Rabbit polyclonal antibody purified with protein G chromatography is supplied in 100μl phosphate-buffered saline with 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects SMAD7 at 46 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY