Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. Regulation of SK1 and SK2 activity may occur through phosphorylation. SK1 is phosphorylated at Ser-225 by ERK leading to increased activity and translocation to the plasma membrane. SK2 is phosphorylated in response to EGF, PKC activators, and phorbol esters. ERK1 can phosphorylate both Ser-351 and Thr-578, and non-phosphorylatable mutants of these sites suppress ERK1-mediated chemotaxis.
Hait, N.C. et al. (2007) J Biol. Chem. 282(16):12058.
Hait, N.C. et al. (2005) J Biol. Chem. 280:29462.
Spiegel, S. & Milstien, S. (2003) Nat. Rev. Mol. Cell Biol. 4:397.
*For more information, see UniProt Accession Q9NRA0
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Xia, C. et al. (2018) Antiviral Res. 158:171. (WB: human A549 cells)
Fu, P. et al. (2016) J Biol Chem. 291(53):27187. (WB: human endothelial cells)
Wang, X. et al. (2016) PLoS Pathog. 12(10):e1005926. (WB: brain microvascular endothial cells)
Wallington, C.T. et al. (2014) Cancer Res(74):2803. (WB: human leukemia cells)
Dai, L. et al. (2013) Mol Endocrin 28(2):197. (WB: MCF7)
This kit contains: