Catalog # SP4461

Semaphorin-3E (N-terminal region) Antibody

Rabbit Polyclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 87 kDa

DATASHEET  




$245

The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3E (Sema-3E) is a class III secreted semaphorin that binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. Mutations in the Sema-3E gene are associated with CHARGE syndrome, a disorder that has nerve and cardiovascular abnormalities. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa). These forms are observed as monomers, as well as homo- and hetero-dimers. Sema-3E protein is frequently expressed in human cancer cell lines and in solid tumors from breast cancer patients. The expression of Sema-3E in mammary adenocarcinoma cells induces the ability to form experimental lung metastasis, and this activity requires furin-dependent production of the 61 kDa Sema-3E protein.

 

References

Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.
Gu, C. et al. (2005) Science. 307(5707):265.
Feiner, L. et al. (1997) Neuron. 19(3):539.

Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Sema-3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Sema-3E (N-terminal region) blocking peptide (lanes 2 & 4).



Sema-3E (N-terminal region) synthetic peptide corresponds to amino acids in the N-terminal region of human Sema-3E. This sequence is well conserved in rat, mouse, and chicken Sema-3E, and has low homology to other semaphorin family members.

*For more information, see UniProt Accession O15041
Rabbit polyclonal, affinity-purified antibody is supplied in phosphate-buffered saline and 0.05% sodium azide. Aliquot and Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Semaphorin 3E (N-terminal region) peptide (without carrier). The antibody detects 87 and 61 kDa bands corresponding to the full-length and furin-proccessed forms of Sema-3E in human PC-3 and rat PC12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY