Catalog # SP3901
Slingshot-1L (Ser-978), phospho-specific
Size 100 μl
Species Reactivity Hu, Rt, Ms, Ck
MW 150 kDa
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Phosphorylation of SSH1L at Ser-937 and Ser-978 by PKD leads to association with 14-3-3, sequestration of the phosphatase to the cytoplasm, and reduces cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.
Wang, Y. et al. (2015) Cancer Lett. 360(2):171 (ICC:PC cell lines, KLM1, KLM1-R, AsPC-1, BxPC-3, PK45p, PK59)
DiPaolo, B.C. et al. (2013) Am Jou of Phys 305(2): L141. (WB: rat alveolar type II)
Soosairajah, J. et al. (2005) EMBO J. 24(3):473.
Endo, M. et al. (2003) J Neurosci. 23(7):2527.
Niwa, R. et al. (2002) Cell 108:233.
*For more information, see UniProt Accession Q8WYL5
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Tedeschi, A. et al. (2019) Neuron. Online Aug. 7. (WB: rat DRG neurons)
Kermath, BA et al. (2019) Cereb Cortex. Apr 11. pii: bhz070. (WB: human brain)
Tan, F. et al. (2015) J Exp Clin Cancer Res. 9(34):117 (WB: HCT-116, LoVo, CRC tissues)
Bencsik, N. et al. (2015) J Cell Biol. 210(5):771. (WB: hippocampal dendritic spines)
Xiu, L. et al. (2015) Am J Pathol. 185(2):387 (WB: human MSCs and HPCs)
Barone, E. et al. (2014) Bioch Biophys Acta 1842(12A):2500. (WB: mouse brain)
Xiang, S.Y. et al. (2013) Sci Signal. 6(306):ra108. (WB: rat cardiomyocytes, S1P treated)
DiPaolo, B.C. et al. (2013) Am Jou of Phys 305(2): L141 (WB: rat alveolar type II)
Wang, Y. et al. (2013) J Neurosci. 33(15):6423. (WB: rat infralimbic cortex)
Vitolo, M. et al. (2013) Oncogene. 32(17):2200. (WB: MCF-10A, 293T)
Sparrow, N. et al. (2012) J Neurosci. 32(15):5284. (WB & ICC: rat Schwann cells)
This kit contains: