Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.
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*For more information, see UniProt Accession Q9NYA1
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Bruno, G. et al. (2019) Oncogene. s41388-019-0993-1. (WB: human neuroblastoma)
Bandara, G. et al. (2019) Front Immunol. 9:631. (WB: human mast cells)
Hanyu, T. et al. (2018) Surgery. 163(6):1301. (IHC: human gastric cancer)
Stayrook, KR et al. (2015) Bonekey Rep. 4:719. (IP: human MDA-MB-231 cells)
Bruno, G et al. (2015) Biochim Biophys Acta. 1851(2):194. (WB: mouse C2C12 myoblasts)
Frati, A. et al. (2015) Mol Endocrinol. 29(1):53. (WB: rat H9c2 cardiomyoblasts, siRNA knockdown)
Yasuo, M. et al. (2013) PLoS One 8(1):e53927. (WB: rat lung)
Yang, L. et al. (2012) Am J Pathol. 181(1):85. (WB, ICC: mouse bone marrow stem cells)
Chakraborty, G. et al. (2012) J Neurochem. 121(5):806. (WB: rat forebrain)
Siow, D. et al. (2010) J Lipid Res. 51:2546. (WB: HeLa SK1 transfectants )
Kim, M. et al. (2010) Am J Nephrol. 31:353. (WB: human HK-2 cells and SK1 transfectants )
Song, J.H. et al. (2010) AJP Renal Phys. 298:1041. (WB: human HK-2 cells)
Tao, R. et al. (2007) Cardiovas. Res. 74:56. (WB: mouse heart, wildtype vs null)
This kit contains: