SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.
Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.
Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
Zhang, J. et al. (2000) Semin. Immunol. 12:361.
*For more information, see UniProt Accession P29350
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Moogk D. et al. (2016) J Immunol. 197(2):644. (WB: mouse T cells)
Choi, Y.R. et al. (2015) Neurobiol Dis. 83: 90. (IF, WB: BV-2, COS-2, rat primary microglia)
Kataoka, T. R. et al. (2015) Int Immunol. 27(2): 95. (WB: mouse BMMCs)
Khan, T. H. et al. (2014) J Immunology 193(7): 3644. (WB: mouse P388D1)
Mizrahi, SA et al. (2014) J Allergy Clin Immun. 134(1):230. (WB: human mast cells)
Kataoka, T. et al. (2013) Sci Rep. 3:2861. (WB: Kasumi-1 cells)
Le Page, A. et al. (2014) Cell Commun Signal. 12:2. (WB, FC: human T-cells activated)
Tibaldi, E. et al. (2014) BBA 1843(2):288. (WB: hepatic stellate cells)
Hebeisen, M. et al.(2013) J Clin Invest. 123(3):1044. (WB: CD8+T and SUP-T1 cells)
Bandyopadhyay, G. et al. (2012) Clin Immunol. 145(1):44. (FC: human T-cells)
Negro, R. et al. (2012) Blood 119(26):6278. (WB: human chronic lymphocytic leukemia)
Aloulou, M. et al. (2012) Blood. 119(13)3084. (WB: rat mast cells)
Tibaldo, E. et al. (2011) Leukemia. 25(11): 1768. (WB: human B-CCL cells)
Xiao, W. et al. (2010) Blood 116(26):6003. (WB: CD34?KSL cells)
Tachdjian et al. (2010) J Aller Clin Imm. 125(5):1128. (WB: mouse macrophages)
Verhagen, A.M. et al. (2009) J. Immunol. 181:2020. (WB: mouse B cells)
This kit contains: