Catalog # SP1571


SHP1 (Tyr-536), phospho-specific

Rabbit Polyclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 68 kDa

$295

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

 

References

Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.
Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
Zhang, J. et al. (2000) Semin. Immunol. 12:361.

Phospho-SHP1 (Tyr-536) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.

*For more information, see UniProt Accession P29350
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-SHP1 (Tyr-536) peptide. The antibody detects a 68 kDa* band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate, but is not observed in control cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Moogk D. et al. (2016) J Immunol. 197(2):644. (WB: mouse T cells)
Choi, Y.R. et al. (2015) Neurobiol Dis. 83: 90. (IF, WB: BV-2, COS-2, rat primary microglia)
Kataoka, T. R. et al. (2015) Int Immunol. 27(2): 95. (WB: mouse BMMCs)
Khan, T. H. et al. (2014) J Immunology 193(7): 3644. (WB: mouse P388D1)
Mizrahi, SA et al. (2014) J Allergy Clin Immun. 134(1):230. (WB: human mast cells)
Kataoka, T. et al. (2013) Sci Rep. 3:2861. (WB: Kasumi-1 cells)
Le Page, A. et al. (2014) Cell Commun Signal. 12:2. (WB, FC: human T-cells activated)
Tibaldi, E. et al. (2014) BBA 1843(2):288. (WB: hepatic stellate cells)
Hebeisen, M. et al.(2013) J Clin Invest. 123(3):1044. (WB: CD8+T and SUP-T1 cells)
Bandyopadhyay, G. et al. (2012) Clin Immunol. 145(1):44. (FC: human T-cells)
Negro, R. et al. (2012) Blood 119(26):6278. (WB: human chronic lymphocytic leukemia)
Aloulou, M. et al. (2012) Blood. 119(13)3084. (WB: rat mast cells)
Tibaldo, E. et al. (2011) Leukemia. 25(11): 1768. (WB: human B-CCL cells)
Xiao, W. et al. (2010) Blood 116(26):6003. (WB: CD34?KSL cells)
Tachdjian et al. (2010) J Aller Clin Imm. 125(5):1128. (WB: mouse macrophages)
Verhagen, A.M. et al. (2009) J. Immunol. 181:2020. (WB: mouse B cells)

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).



This kit contains:

KIT SUMMARY