Catalog # SP1401

Semaphorin-3A (N-terminal) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:400
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 95 kDa

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

References

Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Kolodkin, A.L. et al. (1993) Cell 75:1389.
Luo, Y. et al. (1993) Cell 75:217.

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.

Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (SP1401), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Semaphorin 3A synthetic peptide corresponds to amino acids in the N-terminal region of human Sema3A. This sequence is identical to similar regions in rat and mouse Sema3A, and has low homology to other semaphorin family members.

*For more information, see UniProt Accession Q14563

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Semaphorin 3A (N-terminal region) peptide (without carrier). The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera. This antibody has not been shown to be reactive in neonatal rat brain, but has weak reactivity in adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: