Catalog # SM3731


Syk (Central region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:250

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 72 kDa

Isotype IgG1

$245

Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all hematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igα, TCRζ, CD3ε, FcεRIβ, and FceRIγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains, thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cγ1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways.

 

References

Latour, S. et al. (2001) Curr Opin Immunol. 13:299.
Costello, P.S. et al. (1996) Oncogene. 13(12):2595.

Clone M373 was generated from a sequence corresponding to amino acids in the central region of human Syk. This sequence has high homology to mouse and rat Syk.

*For more information, see UniProt Accession P43405
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 72 kDa* protein corresponding to the molecular mass of Syk on SDS-PAGE immunoblots of adult mouse spleen.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-Syk (Central region) antibody at 1:250.



This kit contains:

KIT SUMMARY