Catalog # SM3711


Syntaphilin (N-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 70 kDa

Isotype IgG1

$245

Synaptic vesicles are organelles situated at the distal terminus of the presynaptic neuron. The exocytosis of these vesicles requires docking at the plasma membrane, priming, and fusion. Fusion is mediated by a complex consisting of membrane components of both the synaptic vesicle and the synaptic plasma membrane. The fusion complex consists of the soluble NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs) that include synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of 25kDa). Syntaxin-1 is a key component of the synaptic vesicle docking/fusion machinery which forms the SNARE complex with SNAP-25 and synaptobrevin. Syntaphilin is a brain-specific membrane-associated protein that can inhibit SNARE complex formation by binding free syntaxin-1.

References

Chen, Y.M. et al. (2009) J Neurosci. 29(30):9429.
Lao, G. et al. (2000) Neuron 25:191.

Clone M371 was generated from a sequence corresponding to amino acids in the N-terminal region of human syntaphilin. This sequence has high homology to mouse and rat syntaphilin.

*For more information, see UniProt Accession O15079
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 70 kDa* protein corresponding to the molecular mass of Syntaphilin on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of adult mouse brain lysate. The blot was probed with mouse monoclonal anti-Syntaphilin (N-terminal region) antibody at 1:250 (lane 1), 1:1000 (lane 2), or 1:2000 (lane 3).



This kit contains:

KIT SUMMARY