Catalog # SM3651


Synapsin I (C-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:50
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 80 kDa

Isotype IgG1

$245

Synapsins are important peripheral membrane proteins involved in synaptic vesicle release during neurotransmission between neurons. Several synapsin variants are generated by alternative splicing of the mRNA from three different genes: synapsin I, II, and III. Synapsins bind to synaptic vesicles via their conserved N-terminal domains. Regulation of synapsins may occur through PKA and CAM kinase I phosphorylation. This regulation occurs concomitantly with membrane excitation and neurotransmitter release into the synaptic cleft. Synapsins bundle actin filaments and interact with multiple calcium binding proteins. Thus, synapsins may crosslink vesicles or other proteins to actin filaments at the presynaptic terminal. In addition, synapsins may interact with each other, since synapsin III has been shown to form heteromultimers with both synapsin I and synapsin II. All synapsins contain a central ATP binding domain and variable C-terminal domains that facilitate distinct regulatory functions.

References

Fdez, E. & Hilfiker, S. (2006) Brain Cell Biol. 35(2-3):107.
Südhof, T.C. (1990) J Biol Chem. 265(14):7849.
Südhof, T.C. et al. (1989) Science. 245(4925):1474.

Clone M365 was generated from a sequence corresponding to amino acids in the C-terminal region of mouse synapsin I. This sequence has high homology to human and rat synpasin I.

*For more information, see UniProt Accession O88935
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects an 80 kDa* protein corresponding to the molecular mass of Synapsin I on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Synapsin I (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).

Immunocytochemical labeling of Synapsin I in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Synapsin I (C-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.



This kit contains:

KIT SUMMARY