Catalog # SM2631

Stat3 (N-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 86 kDa

Isotype IgG1

DATASHEET  




$245

The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat3 is expressed as two variants, Stat3α (86 kDa) and Stat3b (79 kDa) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α (86 kDa) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site.

 

References

Biethahn, S. et al. (1999) Exp. Hematol. 27:885.
Darnell, J.E. (1997) Science 277:1630.
Wen, Z. et al. (1995) Cell 82:241.

Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).



Clone (M263) was generated from a recombinant protein that included amino acid residues in the N-terminal region of human Stat3. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.

*For more information, see UniProt Accession P40763
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects 86 and 79 kDa* bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY