Catalog # SM1631

SHP2 (N-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 72 kDa

Isotype IgG1

DATASHEET  




$245

SHP2 (PTP1D, SH-PTP2, or Syp) is a widely expressed protein-tyrosine phosphatase (PTP) that maintains phosphotyrosine homeostasis during growth factor, cytokine, hormone and antigen receptor signaling. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP2 associates with EGF and PDGF growth factor receptors and is activated after stimulation of these receptors. Activation of SHP-2 and its association with Gab1 is critical for sustained ERK activation downstream of both growth factor and cytokine receptors. In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites. Thus, SHP2 is critical for maintaining phosphotyrosine homeostasis in many cell signaling pathways.

 

References
Maroun, C. R. et al. (2000) Mol. Cell. Biol. 20:8513.
Qu, C.K. (2000) Cell Res. 10:279.
Zhang, S. Q. et al. (2002) Mol. Cell. Biol. 22:4062.

Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).



Clone (M163) was generated from a recombinant protein containing amino acids in the N-terminal region of human SHP2. This sequence is highly conserved in rat and mouse SHP2.

*For more information, see UniProt Accession Q06124
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 72 kDa* protein in human A431 and Jurkat cells, and mouse brain. This antibody does not cross-react with SHP1.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY