Catalog # SM1601


SHP1 (C-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
IP1:100
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 68 kDa

Isotype IgG1

$245

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

 

References

Ravichandran, K.S. (2001) Oncogene 20(44):6322.
Cutler, R.L. et al. (1993) J Biol Chem 268:21463.

Clone (M160) was generated from a recombinant protein containing amino acids in the C-terminal region of human SHP1. This sequence is highly conserved in rat and mouse SHP1.

*For more information, see UniProt Accession P29350
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 68 kDa* protein in human A431 and Jurkat cells, and does not cross-react with SHP2.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of ShcA expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-ShcA (SP1331) at 1:1000 (lane 1) or 1:4000 (lane 2) or mouse monoclonal ShcA (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).



This kit contains:

KIT SUMMARY