Catalog # RS3251

Anti-Rabbit Ig Light-Chain Specific:HRP Secondary Antibody

Mouse Monoclonal

Application Dilution
ELISA1:5000
ICC1:1000
IHC1:1000
WB1:5000

Size 100 μl

Species Reactivity Rb

Isotype IgG1

DATASHEET  




$55

An array of chromogenic, fluorogenic and chemiluminescent substrates are available for use with HRP conjugated secondary antibodies HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. Antibody-HRP conjugates are superior to antibody-AP conjugates with respect to the specific activities of both the enzyme and antibody. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry.

 

Western blot analysis of human A431 cells (lanes 1, 3, & 5) and mouse brain tissue (lanes 2, 4, & 6). The blot was probed with rabbit polyclonal anti-Actin (AP1651), then Mouse anti-Rabbit light chain specific:HRP secondary antibody was used at 1:5,000, 1:10,000, and 1:20,000.



Clone 5A6-1D10 HRP-conjugated mouse monoclonal secondary reagent reacts with the light chains, but not heavy chains from rabbit immunoglobulins, and has minimal cross-reactivity with bovine, goat, horse, human, mouse, rat, and sheep immunoglobulins.

Mouse monoclonal antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, and 1 mg/ml BSA. Store at –20°C. Stable for 1 year. Note: Use of sodium azide as preservative substantially inhibits the enzyme activity of HRP.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody reacts with the light chains of rabbit immunoglobulins in various antibody applications that utilize HRP readouts, such as western blot, ELISA, immunocytochemistry, and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY