Catalog # RP1361
RhoA (Ser-188), phospho-specific Antibody
Rabbit Polyclonal
| ELISA | 1:2000 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 22 kDa
Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDP-bound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.
References
Ellerbroek, S.M. et al. (2003) J. Biol. Chem. 278(21):19023.
Sawada, N. et al. (2001) Biochem. Biophys. Res. Commun. 280:798.
Dong, J.M. et al. (1998) J. Biol. Chem. 273(35):22554.
Lang, P. et al. (1996) EMBO J. 15:510.
Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (RP1501; lanes 1 & 2) or with anti-RhoA (Ser-188) (RP1361; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).
*For more information, see UniProt Accession P06749
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
Giralt, A. et al. (2016) Glia. 64(4):620 (WB: mouse astrocyte)Utsumi, T. et al. (2014)J Urol. 192(2):567 (WB: 786-O cells)
Selimovic, D. et al. (2013) Apoptosis. 18(8):980. (WB: human melanoma cells)
Harma, V. et al. (2012) Oncogene. 31:2075. (WB: human PC-3 cells)
Xu, J. et al. (2011) Int J Cancer. Online Mar 8. (WB: rat lymphatic ECs)
This kit contains:
