Catalog # PP4641


Phosphothreonine

Rabbit Polyclonal

Application Dilution
ELISA1:1000
ICC1:250
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

$245

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

 

References
Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.
Hunter T.(1987) Cell. 50(6):823.
Phosphothreonine synthetic peptides coupled to carrier protein.

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using phospho-threonine peptide (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. The blot was untreated (lane 1) or treated with lambda phosphatase (lanes 2), then probed with anti-Phosphothreonine (PP4641) at 1:1000.

Immunocytochemical labeling of phosphothreonine upregulation in control (left) or calyculin A-treated HeLa cells (right). The cells were labeled with rabbit polyclonal anti-Phosphothreonine (PP4641). The antibody was detected using goat anti-rabbit DyLight® 594.



This kit contains:

KIT SUMMARY