Catalog # PP4501


Paxillin (Tyr-118), phospho-specific

Rabbit Polyclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 68 kDa

$295

Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.

 

References
Schaller, M.D & Schaefer, E.M. (2001) Biochem J. 360:57.
Salgia, R. et al. (1995) J Biol Chem. 270(49):29145.
Phospho-Paxillin (Tyr-118) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 118 from human paxillin. This sequence is highly conserved in rat and mouse paxillin and is also found in all isoforms (a, b, g) of paxillin.

*For more information, see UniProt Accession P49023
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).



This kit contains:

KIT SUMMARY