Catalog # PP3501


p38α MAP Kinase (a.a. 319-328)

Rabbit Polyclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 38 kDa

$245

p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.

 

References
Jirmanova, L. et al. (2009) Blood 113(10):2229.
Salvador, J.M. et al. (2005) Nat Immunol. 6(4):390.
Han, J. et al. (1994) Science 265:808.
Lee, J. C. et al. (1994) Nature 372:739.
p38α MAP Kinase synthetic peptide (coupled to KLH) corresponding to amino acid residues 319 to 328 in mouse p38α. This peptide sequence is highly conserved in human and rat p38α, and has high homology to the conserved site in p38β.

*For more information, see UniProt Accession P47811
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using p38α (a.a. 319-328) peptide (without carrier). The antibody detects a 38 kDa* band corresponding to p38α on SDS-PAGE immunoblots of human Jurkat and K562, as well as mouse macrophage (J774A.1) cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38α (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38α (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).



This kit contains:

KIT SUMMARY