Catalog # PP2551
Size 100 μl
Species Reactivity Hu, Rt, Ms
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
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The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Xue, J. et al. (2019) J Integr Plant Biol. May 14.12824. (WB: Arabidopsis)
Xiao, N. et al. (2019) FASEB J. 33(1):163-174. (IP: Hek293 cells)
Unger, A. et al. (2017) Acta Neuropathol Commun. 5(1):72. (WB: mouse skeletal muscle)
Liu, J. et al. (2016) Cell. 167(4):10521066.e18. (WB: HEK293 transfectants)
Tsai, C.F. et al. (2015) J Agric Food Chem. 9;63(48):10388 (WB: Huh7 cells)
Kommaddi, R.P. et al. (2015) J Biol Chem. 3;290(14):8888 (WB: COS-7 cells)
Kim, SY et al. (2015) Stem Cells Dev. 24(6):686. (WB: mouse stem cells)
Sroyraya, M. (2013) Microc Res Tech 76(1):102. (WB: Swimming Crab)
Kim, S. et al. (2013) Diabetes. 62(2):471. (WB & IP: mouse adipocytes)
Hummel, S. et al. (2012) Appl Environ Microbiol. 78(4):1140. (WB: human colonic adenocarcinoma epithelial cells T84)
Laluk, K. et al. (2011) Plant Cell 23(8):2831. (WB: Arabidopsis BIK1)
This kit contains: