Catalog # PP2411
PTP1B (Ser-50), phospho-specific Antibody
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 50 kDa
PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.
Lingamanaidu, V. et al. (2001) Molecular Endocrinology 15(10):1768.
Rhee, J. et al. (2001) J. Biol. Chem. 276(9):6640.
Bandyopadhyay, D. et al. (1997) J. Biol. Chem. 272(3):1639.
*For more information, see UniProt Accession P18031
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Obanda, D. et al. (2012) Diabetes 61(3):597. (WB: L6 myoblasts)
This kit contains: