Catalog # PP1341
Paxillin (Ser-83), phospho-specific Antibody
Size 100 μl
Species Reactivity Rt, Ms
MW 68 kDa
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both ERK and p38MAPK kinases phosphorylate serine 83 in vitro. HGF stimulation of murine epithelial cells leads to ERK-mediated phosphorylation of Ser-83, which is required for HGF-induced cell spreading and migration. In addition, Ser-83 is phosphorylated in response to NGF in PC12 cells, and this phosphorylation may be involved in neurite extension. In human paxillin, Ser-85 rather than Ser-83 may be the site phosphorylated by p38 MAPK and mutation of this site inhibits NGF-induced neurite extension. Thus, serine residues in the N-terminal region of paxillin may be important for growth-factor mediated changes in activity.
Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
*For more information, see UniProt Accession Q8VI36
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Fan, Y. et al. (2018) Front Cell Neurosci. 12:309. (WB: mouse BV2 microglia)
Fuste, NP et al. (2016) Nat Commun. 7:11581. (WB/ICC/IHC: rat tumor cells)
Wu, D. W. et al. (2014) Oncogene 33(35): 4385. (WB: CCM3 cells)
Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. (WB: rat C6 and human U373 gliomas)
Sen, A. et al. (2012) J Clin Invest. 122(7):2469. (WB, ICC: C4C2, PC3, LNCaP, )
Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. (WB: pancreatic INS-1 cells)
Sen, A. et al. (2010) J Biol Chem. 285(37):28787. (WB: human LNCAP cells)
This kit contains: