Catalog # PM4991

PKC (α,β,γ) Antibody

Mouse Monoclonal

Application / Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 82 kDa

Isotype IgG2a

$245


DATASHEET  

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

 

References
Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Nishizuka, Y. (1988) Nature 334:661.

Western blot analysis of PKC isoforms in adult mouse brain lysate. The blot was probed with mouse monoclonal anti-PKC (α,β,γ) clone M499 at 1:250 (lane 1) and 1:1000 (lane 2).



Clone (M499) was generated from a recombinant human PKCγ that included amino acids residues 499-697.

*For more information, see UniProt Accession P05129
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects 80-82kDa* proteins corresponding to the molecular mass of PKCα, PKCβ, and PKCγ on SDS-PAGE immunoblots of mouse brain lysates. Similar results were observed in human and mouse lysates. This antibody detects recombinant PKCα, PKCβ1, and PKCγ, and possibly detects other PKC isoforms as well.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: