Catalog # PM2691
PYK2 (Tyr-402), phospho-specific Antibody
Mouse Monoclonal
| ELISA | 1:2000 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 116 kDa
Isotype IgG1
Protein tyrosine kinase, Pyk2 (CAKb, RAFTK, CADTK), is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration of various cell types. In T-cells, Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR. Phosphorylation of Tyr-402 is required for the phosphorylation of other tyrosines in Pyk2 and provides a binding site for Fyn SH2 during T-cell activation.
References
Duong, L. T. et al. (2001) J. Biol. Chem. 276:7484.
Katagiri, T. et al. (2000) J. Biol. Chem. 275:19645.
Avraham, S. et al. (1995) J. Biol. Chem. 270:27742.
Lev, S. et al. (1995) Nature 376:737.
Sasaki, H. et al. (1995) J. Biol. Chem. 270:21206.
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2 (Tyr-402) (lanes 3 & 4).
*For more information, see UniProt Accession Q14289
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
