Catalog # PM2341

PTP1B (C-terminal region) Antibody

Mouse Monoclonal

Application / Dilution

Size 100 μl

Species Reactivity Hu

MW 50 kDa

Isotype IgG2a



PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.



Lingamanaidu, V. et al. (2001) Molecular Endocrinology 15(10):1768.
Rhee, J. et al. (2001) J. Biol. Chem. 276(9):6640.
Bandyopadhyay, D. et al. (1997) J. Biol. Chem. 272(3):1639.

Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with mouse monoclonal anti-PTP1B (lanes 1 & 2) or rabbit polyclonal anti-PTP1B (Ser-50) (lanes 3 & 4).

Immunocytochemical labeling of PTP1B in methanol and acetone fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monclonal anti-PTP1B (PM2341) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Clone M234 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PTP1B.

*For more information, see UniProt Accession P18031
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 50 kDa* band corresponding to PTP1B in western blots of human A431, Jurkat, and HeLa cells. It does not detect PTP1B in rat PC12 or mouse SYF cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: